Enhancement of thermal stabilization of formaldehyde dehydrogenase from Pseudomonas putida by directed evolution.

Biosci Biotechnol Biochem

Toyota Central R&D Labs., Inc., Aichi-gun, Aichi.

Published: November 2010

We used directed evolution to enhance the thermostability of formaldehyde dehydrogenase from Pseudomonas putida. At 50 degrees C, the wild-type enzyme was inactivated within 30 min, but the variants obtained retained 80% activity for at least 300 min. At room temperature (30 degrees C), the variants obtained retained <80% activity for at least 500 h (21 d).

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http://dx.doi.org/10.1271/bbb.100026DOI Listing

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