The generation of anaphylatoxins, particularly C5a, is important in extracorporeal circulation therapies such as granulocyte/monocyte apheresis, which activates the complement system and elevates C5a levels. However, no side effects of granulocyte/monocyte apheresis using cellulose acetate beads have been reported. To investigate the mechanism of complement activation, we prepared plasma from cellulose acetate bead-treated blood (P-CAB) and compared it with zymosan-activated plasma (ZAP). Anaphylaxis activity was measured by skin test, and the activity of carboxypeptidase, which inactivates C5a, was measured by colorimetric assay. Pro-carboxypeptidase R and neutrophil elastase concentrations were measured by enzyme-linked immunosorbent assay. Although C5a was generated in P-CAB, the anaphylaxis activity of P-CAB was lower than that of ZAP. Carboxypeptidase activity and pro-carboxypeptidase R levels were suppressed in P-CAB, but not in ZAP. Furthermore, neutrophil elastase levels increased in P-CAB. The decreases in carboxypeptidase activity and inactivation of anaphylatoxin were inhibited by a neutrophil elastase inhibitor. These results suggest that cellulose acetate beads initiate the activation of carboxypeptidase R via elastase release, thereby inducing the inactivation of anaphylatoxin.

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1525-1594.2009.00995.xDOI Listing

Publication Analysis

Top Keywords

cellulose acetate
16
acetate beads
12
neutrophil elastase
12
complement system
8
granulocyte/monocyte apheresis
8
anaphylaxis activity
8
carboxypeptidase activity
8
inactivation anaphylatoxin
8
p-cab
5
activity
5

Similar Publications

Improving CO Removal Efficiency with Bio-Cellulose Acetate: A Multi-Stage Membrane Separation Approach.

Polymers (Basel)

January 2025

Biomass and Oil Palm Research Center of Excellence, Walailak University, Thasala, Nakhon Si Thammarat 80160, Thailand.

In this comprehensive investigation, the sustainable production and utilization of gas separation membranes derived from coconut water (CW) waste was investigated. The research focuses on the synthesis of bacterial cellulose (BC) and cellulose acetate (CA) membranes from CW, followed by a thorough analysis of their characteristics, including morphology, ATR-FTIR spectroscopy, tensile strength, and chemical composition. The study rigorously evaluates membrane performance, with particular emphasis on CO/CH selectivity under various operational conditions, including pressure, membrane thickness, and number of stages.

View Article and Find Full Text PDF

Following implantation, infections, inflammatory reactions, corrosion, mismatches in the elastic modulus, stress shielding and excessive wear are the most frequent reasons for orthopedic implant failure. Natural polymer-based coatings showed especially good results in achieving better cell attachment, growth and tissue-implant integration, and it was found that the inclusions of nanosized fillers in the coating structure improves biomineralization and consequently implant osseointegration, as the nanoparticles represent calcium phosphate nucleation centers and lead to the deposition of highly organized hydroxyapatite crystallites on the implant surface. In this study, magnetic nanoparticles synthesized by the co-precipitation method were used for the preparation of cellulose acetate composite coatings through the phase-inversion method.

View Article and Find Full Text PDF

Efficient and accurate determination of the degree of substitution of cellulose acetate using ATR-FTIR spectroscopy and machine learning.

Sci Rep

January 2025

Institute of Biological and Chemical Systems - Functional Molecular Systems (IBCS-FMS), Karlsruhe Institute of Technology (KIT), Karlsruhe, 76344, Germany.

Multiple linear regression models were trained to predict the degree of substitution (DS) of cellulose acetate based on raw infrared (IR) spectroscopic data. A repeated k-fold cross validation ensured unbiased assessment of model accuracy. Using the DS obtained from H NMR data as reference, the machine learning model achieved a mean absolute error (MAE) of 0.

View Article and Find Full Text PDF

The present study included the environmentally friendly production of stable nickel nanoparticles (NiO NPs) using lemon and tomato, followed by their analysis and evaluation for their antibacterial properties against Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, and Bacillus cereus. The Nickel oxide nanoparticles produced exhibited their maximum absorption at 276 nm in the UV-vis spectrum. The image captured FESEM revealed smooth nanofibers with an average diameter of around 259 ± 3.

View Article and Find Full Text PDF

The objective of this research is to develop a natural macromolecules-based smart double-layer film using carboxymethyl cellulose (CMC) film containing pomegranate peel anthocyanins (PPA) and cellulose acetate nanofibers (CANFs) with Artemisia sieberi Besser essential oil-loaded nanostructured lipid carriers (ABNLCs). Based on the performance as a color indicator, and other studied properties, the CMC/PPA 8 % film was selected as the optimized film. The double-layer film was constructed by electrospinning the CANFs containing ABNLCs on the optimized CMC film.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!