AI Article Synopsis
- A new multiplex PCR assay was created to identify major types and subtypes of staphylococcal cassette chromosome mec (SCCmec) in meticillin-resistant Staphylococcus aureus (MRSA) strains.
- The assay includes a unique 9 valent multiplex PCR along with two primer pairs for accurately detecting S. aureus and its meticillin resistance.
- Testing of 389 Malaysian MRSA isolates and 18 European isolates showed the assay successfully characterized all samples, with SCCmec types III and V being the most common, and an emergence of type IVh noted among Malaysian strains.
Article Abstract
A multiplex PCR assay was developed for the identification of major types and subtypes of staphylococcal cassette chromosome mec (SCCmec) in meticillin-resistant Staphylococcus aureus (MRSA) strains. The method uses a novel 9 valent multiplex PCR plus two primer pairs for S. aureus identification and detection of meticillin resistance. All 389 clinical MRSA isolates from Malaysia and 18 European isolates from the Harmony collection harbouring different SCCmec types that we tested were correctly characterized by our PCR assay. SCCmec type III and V were by far the most common types among both hospital- and community-acquired Malaysian MRSA isolates, with an apparent emergence of MRSA harbouring the IVh type.
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| http://dx.doi.org/10.1099/jmm.0.021956-0 | DOI Listing |
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