AI Article Synopsis
- A novel, fast, and non-destructive method for analyzing the structure of silica-based monolithic columns using confocal laser scanning microscopy is presented.
- This method involves several steps: pretreatment of the column, acquiring images, processing those images, and then conducting statistical analyses on the data.
- The resulting morphological data, particularly the distribution of chord lengths in the macropore space and silica skeleton, is comparable to more complex techniques like transmission electron microscopy but is much easier to implement and access.
Article Abstract
We present a fast, nondestructive, and quantitative approach to characterize the morphology of capillary silica-based monolithic columns by reconstruction from confocal laser scanning microscopy images. The method comprises column pretreatment, image acquisition, image processing, and statistical analysis of the image data. The received morphological data are chord length distributions for the bulk macropore space and skeleton of the silica monolith. The morphological information is shown to be comparable to that derived from transmission electron microscopy, but far easier to access. The approach is generally applicable to silica-based capillary columns, monolithic or particulate. It allows the rapid acquisition of hundreds of longitudinal and cross-sectional images in a single session, resolving a multitude of morphological details in the column.
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| http://dx.doi.org/10.1021/ac100909t | DOI Listing |
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