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Investigating the Role of DNA Ploidy and Proliferation Index in Distinguishing Ameloblastoma from Ameloblastic Carcinoma.
Head Neck Pathol
January 2025
Department of Oral and Maxillofacial Pathology, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.
Purpose: This study aimed to investigate the role of DNA ploidy and proliferation index in distinguishing ameloblastoma (AB) from ameloblastic carcinoma (AC).
Methods: The study included 29 ACs, 6 conventional ABs that transformed into ACs, and a control cohort of 20 conventional ABs. The demographics and clinicopathologic details of the included cases were summarised and compared.
A High-Throughput Integrated Nontargeted Metabolomics and Lipidomics Workflow Using Microelution Enhanced Matrix Removal-Lipid for Comparative Analysis of Human Maternal and Umbilical Cord Blood Metabolomes.
Anal Chem
January 2025
Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hong Kong 999077, China.
Sample pretreatment for mass spectrometry (MS)-based metabolomics and lipidomics is normally conducted independently with two sample aliquots and separate matrix cleanup procedures, making the two-step process sample-intensive and time-consuming. Herein, we introduce a high-throughput pretreatment workflow for integrated nontargeted metabolomics and lipidomics leveraging the enhanced matrix removal (EMR)-lipid microelution 96-well plates. The EMR-lipid technique was innovatively employed to effectively separate and isolate non-lipid small metabolites and lipids in sequence using significantly reduced sample amounts and organic solvents.
View Article and Find Full Text PDFRecent advances in fluorescent gold nanocluster-based bioanalytical analysis and imaging in living cell.
Bioanalysis
January 2025
College of Chemistry, Beijing University of Chemical Technology, Beijing, China.
Ultrasmall and highly fluorescent gold nanoclusters (Au NCs) have been widely used for the construction of sensing and imaging platforms. Specifically, through a combination of surface functionalization and spectral analysis and/or imaging techniques, effective intracellular detection and imaging are realized. In this review, we summarize the recently adopted intracellular analysis and imaging events with Au NCs-based probes.
View Article and Find Full Text PDFCellStudio: a Modular, Tunable and Accessible Platform for Analysis of Growth Factors Secretions in Cell Cultures.
ACS Appl Mater Interfaces
January 2025
Microfluidics Cluster UPV/EHU, BIOMICs Microfluidics Group, University of the Basque Country UPV/EHU, Vitoria-Gasteiz 01006, Spain.
Traditional cell culture methods face significant limitations in monitoring cell secretions with spatial and temporal precision. Advanced microsystems incorporating biosensors have been developed to address these challenges, but they tend to lack versatility, and their complexity, along with the requirement for specialized equipment, limits their broader adoption. CellStudio offers an innovative, user-friendly solution that exploits Printing and Vacuum Lithography combined with bead-based assays to create modular and tunable cell patterns surrounded by biosensors.
View Article and Find Full Text PDFHigh-resolution 0.25 mm Detector CT Has Limited Impact on Right Adrenal Vein Detectability in Preprocedural Contrast Enhanced CT for Adrenal Venous Sampling.
J Comput Assist Tomogr
January 2025
Department of Radiology, University of Yamanashi, Chuo, Yamanashi.
Objective: This study aims to identify factors associated with the detectability of the right adrenal vein (RAV) on preoperative contrast-enhanced CT scans of adrenal venous sampling (AVS) in the era of high-resolution CT (HRCT).
Materials And Methods: In this retrospective study, 36 patients (15 men and 21 women; mean age, 56 y) who underwent preoperative contrast-enhanced CT [11 patients in HRCT with 0.25 mm detector matrix (Cannon Medical Systems) and 25 patients in conventional multidetector CT with 0.
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