A model for the fluorescence sensing properties of small-core high-refractive-index fibers (optical nanowires) is developed and compared quantitatively with experiment. For the first time, higher-order modes and loss factors relevant to optical nanowires are included, which allows the model to be compared effectively with experiment via the use of fluorophore filled suspended optical nanowires. Numerical results show that high-index materials are beneficial for fluorescence-based sensing. However, both numerical and experimental results show that the fluorescence signal is relatively insensitive to core size, except for low concentration sensing where nanoscale fiber cores are advantageous due to the increased evanescent field power.
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