AI Article Synopsis
- - A novel scanless two-photon excited fluorescence microscope is introduced, utilizing selective plane illumination microscopy (SPIM) for enhanced imaging.
- - The system showcases its ability to perform optical sectioning, allowing for detailed imaging at different depths.
- - Depth-resolved imaging has been successfully applied to visualize cameleon protein in the pharyngeal muscle of C. elegans, highlighting its practical applications in biological research.
Article Abstract
We demonstrate a simple scanless two-photon (2p) excited fluorescence microscope based on selective plane illumination microscopy (SPIM). Optical sectioning capability is presented and depth-resolved imaging of cameleon protein in C. elegans pharyngeal muscle is implemented.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1364/OE.18.008491 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Single-shot higher-order transport-of-intensity quantitative phase imaging (SHOT-QPI) is proposed to realize simple, in-line, scanless, and single-shot QPI. However, the light-use efficiency of SHOT-QPI is low because of the use of an amplitude-type computer-generated hologram (CGH). Although a phase-type CGH overcomes the problem, the accuracy of the measured phase is degraded owing to distortion of the defocused intensity distributions, which is caused by a quantization error of the CGH.
View Article and Find Full Text PDFOptical feedback in lasers is being used for unconventional imaging of fluid dynamics, pressure fields, material properties, and free-carrier distribution, especially in spectral regions where two-dimensional detectors are not yet available. As this technique requires scanning the laser spot across the target, the resulting image contrast is often hampered by the speckle effect. Compressed sensing is becoming a workhorse technique for signal analysis, allowing the reconstruction of complex images from a relatively small number of integrated (single-pixel) measurements, and is being efficiently adapted to a number of single-pixel detector cameras.
View Article and Find Full Text PDFTwo dimensional non-scanning transform-free spatial-domain optical coherence tomography.
Biomed Opt Express
November 2019
Department of Physics, Chung Yuan Christian University, Taoyuan 32023, Taiwan.
A novel optical coherence tomography system that can perform scanless two dimensional imaging without Fourier transform is proposed and demonstrated. In the system, a convex cylindrical mirror generates an extended spatial distribution of optical delay in the reference arm and a cylindrical lens is used to form a focused line beam in the sample arm. A charge-coupled device camera captures the two dimensional tomographic image of a sample in a snap-shot manner.
View Article and Find Full Text PDFHybrid 3D ranging and velocity tracking system combining multi-view cameras and simple LiDAR.
Sci Rep
March 2019
SUPA, School of Physics and Astronomy, University of Glasgow, Glasgow, G12 8QQ, UK.
Scanning our surroundings has become one of the key challenges in automation. Effective and efficient position, distance and velocity sensing is key to accurate decision making in automated applications from robotics to driverless cars. Light detection and ranging (LiDAR) has become a key tool in these 3D sensing applications, where the time-of-flight (TOF) of photons is used to recover distance information.
View Article and Find Full Text PDFIntensity-interferometric spectral-domain optical coherence tomography with dispersion cancellation.
J Opt Soc Am A Opt Image Sci Vis
February 2014
We describe a technique for cancelling group-velocity dispersion in spectral-domain (SD) optical coherence tomography (OCT) based on classical intensity correlations. As a classical analogue of quantum OCT, a Hong-Ou-Mandel interferometer is combined with a conventional SD-OCT setup, and correlations between different spectral intensities are calculated. It is shown theoretically that a simple computational procedure used in SD-OCT enables scanless cross-sectional imaging with both dispersion cancellation and a factor-of-√2 resolution enhancement.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!