Tetrahymena thermophila (T. thermophila) BF(5) produces heat through growth and metabolism. By microcalorimetry, the power-time curves of the metabolism of T. thermophila BF(5) at 28 °C were measured and some quantitative parameters were obtained from these curves. Then the action of berberine on this microbe was investigated. Furthermore, the minimum inhibitory concentration (MIC) of berberine against T. thermophila BF(5) growth was obtained by tube dilution method. Berberine of different concentrations had various actions on T. thermophila BF(5) growth: a low concentration (25 μg/ml) of berberine began to inhibit the growth of T. thermophila BF(5) and a high concentration (450 μg/ml) of berberine completely inhibited T. thermophila BF(5) growth. The toxic action of berberine could also be expressed as half-inhibitory concentration IC(50), i.e., 50% effective in this inhibition. The value of IC(50) was 175.60 μg/ml, while the MIC of this compound against T. thermophila BF(5) was 20.76 mg/ml. Berberine has strong toxic action on T. thermophila BF(5) growth. The microcalorimetric method for the assay of toxic action is quantitative, inexpensive and versatile.
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Article Synopsis
- - The study used microcalorimetry to analyze heat flux and metabolism in Tetrahymena thermophila BF5, while assessing the effects of lanthanum (La(3+)) and calcium (Ca(2+)).
- - Low concentrations of La(3+) boosted T. thermophila's metabolism, but higher concentrations inhibited it, with an IC(50) value of 7.2 x 10(-4) mol/l; in contrast, Ca(2+) showed no significant metabolic stimulation at low levels, with a much higher IC(50).
- - Morphological changes in T. thermophila were noted under high doses of La(3+) and Ca(2+), which correlated
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