AI Article Synopsis
- The chemotherapeutic effectiveness of 5'-fluorouracil is diminished due to its breakdown into 2'-fluoro-beta-alanine (FBAL), with dihydropyrimidine dehydrogenase (DPD) being the key enzyme in this process.
- A new HPLC method was created to measure DPD activity and quantify FBAL, which simplifies the testing by not requiring multiple columns or radiolabeled materials.
- The method showed consistent results when calibrating FBAL levels and was effectively utilized to analyze DPD activity in cultured cells.
Article Abstract
The efficacy of the chemotherapeutic drug 5'-fluorouracil is reduced by catabolism to 2'-fluoro-beta-alanine (FBAL), a three-step reaction in which dihydropyrimidine dehydrogenase (DPD) catalyzes the rate-limiting step. To study in vitro DPD activity, we developed and validated an isocratic, reverse-phase HPLC method to detect and quantify FBAL without using multiple columns or radiolabeled substrates. Pre-column derivatization of FBAL was performed using o-phthalaldehyde in the presence of two sulfur donors, ethanthiol or beta-mercaptoethanol, and the resulting products assayed. Calibration curves were linear over a range of 10-200 microg/ml and the method was successfully applied to the examination of DPD activity in cultured cells.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2902665 | PMC |
| http://dx.doi.org/10.1016/j.jchromb.2010.05.010 | DOI Listing |
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