AI Article Synopsis
- Computational analysis revealed that common FIV strains have a rev response element (RRE) characterized by a specific RNA secondary structure with six key stem-loop sub-domains, particularly stem-loop A (SLA).
- Mutational studies on both a FIV molecular clone and a reporter system showed that most stems within SLA (SA1-5) are essential for FIV replication, while SA6 is not critical.
- A specific mutation in the FIV-RRE led to decreased viral activity and expression, indicating that the RNA structure is more influential than an antisense protein in driving virus replication.
Article Abstract
Computational analysis of feline immunodeficiency virus (FIV) RNA sequences indicated that common FIV strains contain a rev response element (RRE) defined by a long unbranched hairpin with 6 stem-loop sub-domains, termed stem-loop A (SLA). To examine the role of the RNA secondary structure of the RRE, mutational analyses were performed in both an infectious FIV molecular clone and a FIV CAT-RRE reporter system. These studies disclosed that the stems within SLA (SA1, 2, 3, 4, and 5) of the RRE were critical but SA6 was not essential for FIV replication and CAT expression. These studies also revealed that the secondary structure rather than an antisense protein (ASP) mediates virus expression and replication in vitro. In addition, a single synonymous mutation within the FIV-RRE, SA3/45, reduced viral reverse transcriptase activity and p24 expression after transfection but in addition also showed a marked reduction in viral expression and production following infection.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2902707 | PMC |
| http://dx.doi.org/10.1016/j.virol.2010.04.009 | DOI Listing |
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