AI Article Synopsis
- Carvacrol and mild heat treatment were tested to see if they could increase the effectiveness of gamma-irradiation on Bacillus cereus bacteria in broth.
- The study examined how these treatments affected various factors, including bacterial viability, cell morphology, membrane fatty acid composition, ATP levels, and DNA degradation.
- Results indicated that combining carvacrol or heat with gamma-irradiation had stronger effects than using radiation alone, leading to greater damage to the bacteria's cell membrane and enhanced DNA degradation.
Article Abstract
Carvacrol and mild heat treatment were tested for their efficiency to increase the radiosensitivity of Bacillus cereus in broth. The bacterium was treated with gamma-irradiation alone or in combination with carvacrol at its minimal inhibitory concentration or mild heat treatment for 10 min at 45 degrees C. The effects of this combination of treatments were studied on various parameters: the bacterial viability, the modifications of the cell morphology with scanning electron microscopy (SEM), the cellular fatty acids composition of the membrane quantified by gas chromatography, the intracellular and extracellular adenosine 5'-triphosphate (ATP) concentrations, and the DNA degradation. Combined treatments resulted in additive or synergistic effects as compared to gamma-irradiation alone. A significant modification (P < or = 0.05) of the fatty acid composition and unsaturation ratios was observed. Pretreatment with mild heat or carvacrol before irradiation disturbed the membrane integrity of B. cereus and induced a significant decrease (P < or = 0.05) of the intracellular ATP concentration. SEM observations revealed that the cell membrane was more severely affected with combined treatment than irradiation alone. The electrophoresis analysis showed that DNA degradation by combined treatments was greater than the gamma-irradiation alone.
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| http://dx.doi.org/10.1021/jf101044f | DOI Listing |
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