AI Article Synopsis
- - A study created knockout mice by disrupting 472 genes related to secreted and membrane proteins to better understand gene functions and their role as drug targets.
- - These knockout mice were systematically tested to observe changes in key areas like embryonic development, metabolism, and various body systems (immune, nervous, cardiovascular).
- - This research represents a unique effort to comprehensively assess the phenotypes of many mouse mutants created through gene-specific methods, which hasn't been done before.
Article Abstract
Large collections of knockout organisms facilitate the elucidation of gene functions. Here we used retroviral insertion or homologous recombination to disrupt 472 genes encoding secreted and membrane proteins in mice, providing a resource for studying a large fraction of this important class of drug target. The knockout mice were subjected to a systematic phenotypic screen designed to uncover alterations in embryonic development, metabolism, the immune system, the nervous system and the cardiovascular system. The majority of knockout lines exhibited altered phenotypes in at least one of these therapeutic areas. To our knowledge, a comprehensive phenotypic assessment of a large number of mouse mutants generated by a gene-specific approach has not been described previously.
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| http://dx.doi.org/10.1038/nbt.1644 | DOI Listing |
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