AI Article Synopsis
- The study aimed to create and validate a user-friendly microarray system for tracking microbial communities in groundwater during various stages of remediation processes.
- The microarray can detect around 100-1,000 cell equivalents of DNA and provides results within 4 hours in the field.
- Analysis of 50 samples showed consistent changes in microbial signatures correlating with environmental changes, confirming the microarray's accuracy in assessing microbial responses in uranium-contaminated sites.
Article Abstract
The objective of this study was to develop and validate a simple, field-portable, microarray system for monitoring microbial community structure and dynamics in groundwater and subsurface environments, using samples representing site status before acetate injection, during Fe-reduction, in the transition from Fe- to SO(4)(2-)-reduction, and into the SO(4)(2-)-reduction phase. Limits of detection for the array are approximately 10(2)-10(3) cell equivalents of DNA per reaction. Sample-to-answer results for the field deployment were obtained in 4 h. Retrospective analysis of 50 samples showed the expected progression of microbial signatures from Fe- to SO(4)(2-) -reducers with changes in acetate amendment and in situ field conditions. The microarray response for Geobacter was highly correlated with qPCR for the same target gene (R(2) = 0.84). Microarray results were in concordance with quantitative PCR data, aqueous chemistry, site lithology, and the expected microbial community response, indicating that the field-portable microarray is an accurate indicator of microbial presence and response to in situ remediation of a uranium-contaminated site.
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| http://dx.doi.org/10.1021/es1006498 | DOI Listing |
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