Objective: We isolated bacterial strain named M-1 from ill Sciaenops ocellatus with ulceration, and further analyzed the phylogenetics of the bacterial strain.

Methods: We characterized the strain on the basis of its morphology, physiology, and sequence comparison of 16SrRNA and HSP60 genes.

Results: Strain M-1 was identified as Vibrio splendidus. 16SrRNA gene of strain M-1 and Vibrio splendidus (AJ874361) had nearest kinship, and the analysis of HSP60 gene sequence indicated that strain M-1 and Vibrio (EU653883, AY837440) located in the same phylogenetic tree.

Conclusion: The results suggested that strain M-1 was a member of Vibrio splendidus.

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