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[Effects of light on flavonoids accumulation and related gene expression in suspension cultures of Scutellaria baicalensis]. | LitMetric
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[Effects of light on flavonoids accumulation and related gene expression in suspension cultures of Scutellaria baicalensis].

Shunqin Chen Yuan Yuan Yujiang Luo Luqi Huang Ping Chen Xiaoming Li

Zhongguo Zhong Yao Za Zhi

Wuhan Polytechnic University, Wuhan 430023, China.

Published: March 2010

Objective: To investigate the effects of light on baicalin,baicalein accumulation and related genes expression in suspension cultures of Scutellaria baicalensis.

Method: The content of baicalin, baicalein in suspension cultures of S. baicalensis was determined by HPLC. The related genes expression was analyzed by semi-quantitative PCR.

Result: Active ingredients in suspension cultures of S. baicalensis were promoted significantly by light, PAL gene transcription under the conditions of light was higher than that under the dark conditions, the UBGAT gene expression levels was significantly related to the baicalein (r = -0.995).

Conclusion: Active ingredients and related genes (PAL, UBGAT) in suspension cultures of S. baicalensis were promoted significantly by light.

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