Objective: To investigate the inhibition of hTERT gene to Hep-2 cells of laryngeal neoplasms.
Method: siRNA-hTERT mRNA was designed. Hep-2 cells, cultured in vitro, were transfected with lipofectamine 2000 and the inhibitory effect was detected by reverse transcriptase (RT-PCR), MTT assay, and morphology.
Result: 1. siRNA were transfected into Hep-2 cells successfully; 2. RT-PCR showed the exogenous hTERT was knocked down 70% by siRNA; 3. The morphology and the MTT result showed the hTERT siRNA to be able effectively to suppress the Hep-2 cell multiplication, the suppression rate achieves 65%.
Conclusion: siRNA target to hTERT can remarkably suppress the proliferation of Hep-2 cell.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Investigation of choline-binding protein of CbpD in the pathogenesis of type 2.
Front Vet Sci
December 2024
College of Medicine, Yichun University, Yichun, China.
serotype 2 ( type 2, SS2) is one of the zoonotic pathogens known to induce meningitis, septicemia, and arthritis in both pigs and humans, resulting in public health concerns. CbpD, also termed CrfP, is one of the choline-binding proteins (CBPs) that was found as a murein hydrolase in SS2 and plays crucial roles in natural genetic transformation under the control of ComRS-ComX regulatory system by a previous study. Nonetheless, the possible functions of CbpD in virulence and pathogenesis in SS2 remain unclear.
View Article and Find Full Text PDFInteraction of Human Respiratory Syncytial Virus (HRSV) Matrix Protein with Resveratrol Shows Antiviral Effect.
Int J Mol Sci
November 2024
Department of Physics, Institute of Biosciences, Humanities and Exact Sciences, São Paulo State University (UNESP), Rua Cristóvão Colombo, 2265, São José do Rio Preto 15054-000, SP, Brazil.
The respiratory syncytial virus (RSV) matrix protein plays key roles in the virus life cycle and is essential for budding, as it stimulates the optimal membrane curvature necessary for the emergence of viral particles. Resveratrol, a polyphenol (3,4',5-trihydroxy-trans-stilbene) produced by plants, exhibits pharmacological effects, including anti-inflammatory and antiviral activities. In this study, resveratrol was tested in HEp-2 (Epidermoid carcinoma of the larynx cell) cells for its post-infection effects, and recombinant M protein was produced to characterize the biophysical mechanisms underlying this interaction.
View Article and Find Full Text PDFEvaluation of the simultaneous effects of KRAS G12V and LCS6 alterations on the behavior of head and neck squamous cell carcinoma.
Mutat Res
December 2024
Selcuk University, Faculty of Medicine, Department of Medical Genetics, Konya, Turkey. Electronic address:
Background: Head and neck squamous cell carcinomas are the seventh most common cancer accounting for 90 % of malignant neoplasia of the upper respiratory system. KRAS is a very important oncogene, leading to the suppression of apoptosis, and promoting the pathogenesis and development of tumors. MicroRNAs (miRNAs) are highly conserved, small noncoding RNA molecules aberrantly expressed in various pathologies including regulation of tumor and metastasis-associated genes.
View Article and Find Full Text PDFAutomatic classification of HEp-2 specimens by explainable deep learning and Jensen-Shannon reliability index.
Artif Intell Med
November 2024
Department of Electronic Engineering, University of Rome Tor Vergata, via del Politecnico 1, 00133 Rome, Italy; Interdisciplinary Center for Advanced Studies on Lab-on-Chip and Organ-on-Chip Applications (ICLOC), University of Rome Tor Vergata, 00133 Rome, Italy.
The Anti-Nuclear Antibodies (ANA) test using Human Epithelial type 2 (HEp-2) cells in the Indirect Immuno-Fluorescence (IIF) assay protocol is considered the gold standard for detecting Connective Tissue Diseases. Computer-assisted systems for HEp-2 image analysis represent a growing field that harnesses the potential offered by novel machine learning techniques to address the classification of HEp-2 images and ANA patterns. In this study, we introduce an innovative platform based on transfer learning with pre-trained deep learning models.
View Article and Find Full Text PDFGolimumab-Induced Anti-NuMA-1 (Nuclear Mitotic Apparatus Protein 1) Antibodies in a Rheumatoid Arthritis Patient: A Case Report.
Cureus
November 2024
Department of Rheumatology B, Ayachi Hospital, Ibn Sina Hospital Center, Faculty of Medicine and Pharmacy, Mohammed V University, Rabat, MAR.
Anti-nuclear mitotic apparatus (NuMA) 1 antibodies are uncommonly detected in routine antinuclear antibody (ANA) screening. We present the case of a 65-year-old female with rheumatoid arthritis undergoing golimumab biotherapy who developed lupus-like symptoms including photosensitivity, fatigue, weakness, myalgias, alopecia, oral ulcers, and worsening of arthritis. Elevated serum levels of NuMA-1 antibodies were detected using indirect immunofluorescence (IIF) on HEp-2 cells with a titer of 1:1000, but no other ANA patterns were associated.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!