Objective: To observe the effect of Naoling decoction on hippocamal histomorphology and the expression of amyloid precursor protein (APP) in CA3 region in rats with Alzheimer's disease (AD), and to explore the therapeutical and the potential mechanism.
Methods: Forty SD rats were classified into 5 groups: a normal group, a sham-operated group, an AD group, a Naoling decoction group, and a Naofukang group. Alzheimer's disease model was established by Abeta1-42 injected into the hippocamal in the rats. The faculty of learning and memory was evaluated by Morris water maze. The changes of cell morphology were detected by HE staining. Expression of APP in CA3 region was mea-sured with immunohistochemical staining.
Results: Morris water maze experiment showed that the escape latency of hidden platform in the AD group were delayed significantly (P<0.05) and the average times of passing was decreased (P<0.05). In the hippocampal CA3 field of the AD rats, HE staining and immunohistochemical test showed that pyramidal cells disturbed, neurons decreased significantly and expression of APP protein increased (P<0.05). Naoling decoction treatment improved pyramidal cellular disorders and decreased APP expression.
Conclusion: Naoling decoction can distinctly improve the learning and memory ability, and reduce the expression of APP in the AD model rats, suggesting that Naoling decoction can be used for the treatment of AD in rats.
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| http://dx.doi.org/10.3969/j.issn.1672-7347.2010.05.005 | DOI Listing |
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Naoling decoction restores cognitive function by inhibiting the neuroinflammatory network in a rat model of Alzheimer's disease.
Oncotarget
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Department of Integrated Traditional Chinese and Western Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China.
Neuroinflammation is central to the pathogenesis of Alzheimer's disease (AD). We previously showed that Naoling decoction (NLD), a traditional Chinese medicine, was effective against AD, acting by inhibiting expression of IL-1β and IL-6. In the present study, we generated the rat model of AD by injecting Aβ1-42 peptide intracerebroventricularly and evaluated the dose-dependent effects of NLD treatment.
View Article and Find Full Text PDFEffect of Naoling decoction on the expression of microglia and IL-6 in hippocampal CA3 region of rats with synthetic Alzheimer's disease.
Zhong Nan Da Xue Xue Bao Yi Xue Ban
February 2013
Department of Traditional Chinese Medicine, Central South University, Changsha, China.
Objective: To observe the effect of Naoling decoction on the learning and memory behaviors and the expression of microglia and IL-6 in hippocampal CA3 region of rats with Alzheimer's disease (AD), and to elucidate the potential mechanism.
Methods: Thirty SD rats were randomly divided into 5 groups: a normal group, a sham-operation group, an AD group, a Naoling decoction group and a Naofukang group. The spatial learning and memory behaviors of the rats were investigated by water maze and Y-maze.
Effect of Naoling decoction on iNOS expression in hippocampal CA3 region in rats with synthetic Alzheimer's disease.
Zhong Nan Da Xue Xue Bao Yi Xue Ban
September 2010
Department of Traditional Chinese Medicine, Second Xiangya Hospital, Central South University, Changsha, China.
Objective: To observe the effect of Naoling decoction on the expression of iNOS in hippocamal CA3 region in rats with Alzheimer's disease (AD), and to explore the potential mechanism.
Methods: Forty SD rats were classified into 5 groups: a normal group, a sham-operated group, an AD group, a Naoling decoction group, and a Naofukang group. The Alzheimer's disease model was established by injecting Aβ1-42 into the hippocamal in the rats.
Effect of Naoling decoction on the expression of APP in hippocampal CA3 region in rats with synthetic Alzheimer's disease.
Zhong Nan Da Xue Xue Bao Yi Xue Ban
May 2010
Department of Traditional Chinese Medicine, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Objective: To observe the effect of Naoling decoction on hippocamal histomorphology and the expression of amyloid precursor protein (APP) in CA3 region in rats with Alzheimer's disease (AD), and to explore the therapeutical and the potential mechanism.
Methods: Forty SD rats were classified into 5 groups: a normal group, a sham-operated group, an AD group, a Naoling decoction group, and a Naofukang group. Alzheimer's disease model was established by Abeta1-42 injected into the hippocamal in the rats.
[Effect of naoling decoction on the behavior and the mRNA expression of beta-amyloid precursor protein in the hippocampus in rats with Alzheimer's disease].
Zhong Nan Da Xue Xue Bao Yi Xue Ban
February 2006
Department of Traditional Chinese Medicine, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Objective: To observe the effect of naoling decoction (NLD) on the behavior and the mRNA expression of beta-amyloid precursor protein (APP) in the hippocampus in rat model with Alzheimer's disease (AD).
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