Using an EZ-Step MRSA rapid kit, a novel screening test for methicillin-resistant Staphylococcus aureus (MRSA) that detects penicillin-binding protein 2a, 34 of 36 MRSA-positive clinical blood culture samples were positive on direct testing (sensitivity, 94.4%), whereas 21 of 21 methicillin-susceptible S. aureus-positive samples were negative (specificity, 100%).
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http://dx.doi.org/10.1016/j.diagmicrobio.2010.02.018 | DOI Listing |
Sci Rep
January 2025
Departamento de Microbiologia Médica, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21951-902, Brazil.
Staphylococcus aureus is a relevant pathogen in bloodstream infections (BSI), and the emergency of the COVID-19 pandemic increased its antimicrobial resistance. S. aureus isolates from BSI (September/2019 - March/2021) were analyzed phenotypically and molecularly, in addition to the clinical features of the patients.
View Article and Find Full Text PDFEcotoxicol Environ Saf
January 2025
Department of Medical Microbiology, Second Faculty of Medicine, Charles University and Motol University Hospital, Czech Republic. Electronic address:
Public transport represents a potential site for the transmission of resistant pathogens due to the rapid movement of large numbers of people. This study aimed to investigate the bacterial contamination of frequently touched surfaces in the public transport system operating in the proximity of the biggest Czech hospital during the coronavirus pandemic despite extensive cleaning and disinfection efforts. In June and September 2020, samples from the metro trains, ground transport and stationary objects were collected, enriched and cultured.
View Article and Find Full Text PDFInfect Drug Resist
December 2024
Respiratory Support Team, Saitama Medical University International Medical Center, Hidaka, Saitama, Japan.
Background: Ventilator-associated pneumonia (VAP) is one of the most lethal complications in intensive care unit (ICU) patients. However, critical issues of non-survivors vary and are still unclear in VAP patients.
Methods: The clinical differences between survivors and non-survivors of VAP were retrospectively analyzed in patients hospitalized from April 2023 to March 2024.
Sci Rep
January 2025
Department of Pharmaceutical Microbiology and Microbiological Diagnostic, Medical University of Lodz, Łódź, Poland.
The treatment of infections caused by Staphylococcus hominis remains a challenge, mainly due to the increasing resistance of these bacteria to antibiotics. The aim of the study was to determine antibiotic resistance in 62 strains S. hominis isolated from clinical materials, and to identify the molecular basis of resistance to antibiotics.
View Article and Find Full Text PDFSci Rep
January 2025
Department of Biological and Medical Sciences, Charles University, Faculty of Pharmacy in Hradec Králové, 2089, Zborovská, Hradec Králové, 500 03, Czech Republic.
Polymicrobial biofilms, the reason for most chronic wound infections, play a significant role in increasing antibiotic resistance. The in vivo effectiveness of the new anti-biofilm therapy is conditioned by the profound evaluation using appropriate in vitro biofilm models. Since nutrient availability is crucial for in vitro biofilm formation, this study is focused on the impact of four selected cultivation media on the properties of methicillin-resistant Staphylococcus aureus and Candida albicans dual-species biofilms.
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