It is shown that Methyl Red can be used as an indicator dye that changes color in Escherichia coli culture as a result of time- and cell density-dependent bleaching by azoreductase produced by the bacteria. For cell cultures that are being used to express a recombinant protein, this phenomenon can be exploited to provide a simple visual cue that cell cultures have reached an appropriate growth phase for addition of an agent to induce protein expression, such as isopropyl thiogalactoside.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2896908 | PMC |
| http://dx.doi.org/10.1021/bi1007194 | DOI Listing |
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