AI Article Synopsis
- Inherited arrhythmias linked to mutations in the RyR2 receptor have been studied in the RyR2(R4496C) mouse model, where abnormal calcium release during diastole is observed in ventricular myocytes.
- Recent optical mapping shows that the His-Purkinje system, rather than ventricular myocytes, may be the main source of these arrhythmias.
- The study reveals that Purkinje cells from this model exhibit significantly higher spontaneous calcium release rates and amplitudes compared to ventricular myocytes, suggesting they are more impacted by the RyR2(R4496C) mutation.
Article Abstract
Background: Inherited arrhythmias can be caused by mutations in the cardiac ryanodine receptor (RyR2). The cellular source of these arrhythmias is unknown. Isolated RyR2(R4496C) mouse ventricular myocytes display arrhythmogenic activity related to spontaneous Ca(2+) release during diastole. On the other hand, recent whole-heart epicardial and endocardial optical mapping data demonstrate that ventricular arrhythmias in the RyR2(R4496C) mouse model of catecholaminergic polymorphic ventricular tachycardia (CPVT) originate in the His-Purkinje system, suggesting that Purkinje cells, and not ventricular myocytes, may be the cellular source of arrhythmogenic activity. The relative effect of the RyR2(R4496C) mutation on calcium homeostasis in ventricular myocytes versus Purkinje cells is unknown.
Objective: This study sought to determine which cardiac cell type is more severely affected, in terms of calcium handling, by expression of the RyR2(R4496C) mutant channel: the ventricular myocytes or the Purkinje cells.
Methods And Results: To discriminate Purkinje cells from ventricular myocytes, we crossed the RyR2(R4496C) mouse model of CPVT with the Cx40(EGFP/+) transgenic mouse. This genetic cross yields Purkinje cells that express eGFP, and therefore fluoresce green when excited by the appropriate wavelength; ventricular myocytes, which do not express connexin 40, are not green. Intracellular calcium was measured in each cell type using calcium-sensitive probes. Purkinje cells of the RyR2(R4496C) mouse model of CPVT show an approximately 2x greater rate (P < .05) and approximately 2x to 3x greater amplitude (P < .000001) of spontaneous calcium release events than ventricular myocytes isolated from the same heart.
Conclusion: These results demonstrate that focally activated arrhythmias originate in the specialized electrical conducting cells of the His-Purkinje system in the RyR2(R4496C) mouse model of CPVT.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2910215 | PMC |
| http://dx.doi.org/10.1016/j.hrthm.2010.06.010 | DOI Listing |
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