AI Article Synopsis
- A 407 bp nucleotide sequence named NTPp13 was isolated from tobacco, showing high similarity to a maize pollen-specific expression promoter, Zm13.
- NTPp13 was fused with the GUS reporter gene and used to create transgenic tobacco plants, where it effectively directed GUS expression specifically in anthers, pollen, and pollen tubes.
- The results indicated that NTPp13 is likely a crucial component of a promoter region for a yet-to-be-identified pollen-specific gene in tobacco, maintaining stable expression under varying temperatures.
Article Abstract
A novel 407 bp nucleotide sequence NTPp13 was isolated from tobacco (Nicotiana tabacum L.) by PCR, its structure and function were characterized. The NTPp13 sequence was highly homologous with the pollen-specific expression promoter Zm13 from maize (Zea mays L.) and contained some key motifs which controlled pollen-specific expression. The NTPp13 was fused to the beta-glucuronidase (GUS) reporter gene and transferred into tobacco. Analysis of the transgenic plants revealed that this putative promoter fragment was sufficient to direct GUS expression specifically in the anther, exactly in the pollen and pollen tube, and that GUS activity reached the maximum at the stage of pollen grain began to separate. Further study showed that the expression of NTPp13 sequence at pollen was stable at the range of temperature measured. These data suggested that the NTPp13 sequence was likely the essential element of promoter region of an unknown pollen-specific gene from tobacco.
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