AI Article Synopsis
- Cloning of the pceA gene, responsible for breaking down tetrachloroethene (PCE), was done using environmental DNA.
- Two related genes were amplified using PCR, but efforts to express them in E. coli did not succeed.
- However, PceA1, produced in vitro, was enzymatically active and formed a complex with the host's DnaK, leading to abnormal filamentous growth in the recombinant E. coli.
Article Abstract
Cloning of pceA, the gene of tetrachloroethene (PCE)-reductive dehalogenase, was undertaken from environmental DNA. Two genes were amplified using PCR primer deduced from pceA. Functional expression of these genes was unsuccessful in Escherichia coli, but PceA1 synthesized in vitro was enzymatically active. In recombinant E. coli, PceA1 formed a complex with host DnaK and caused filamentous growth.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1271/bbb.100027 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The Association of Mitochondrial tRNA G5783A Mutation with Major Depressive Disorder in Two Han Chinese Families.
Neuropsychiatr Dis Treat
January 2025
Department of Psychiatry, Suzhou Guangji Hospital, Suzhou, People's Republic of China.
Objective: In this study, we examined the genetic, medical, and molecular traits of two Han Chinese families with the tRNA G5783A mutation to investigate the relationship between mitochondrial DNA (mtDNA) mutations and major depressive disorder (MDD).
Methods: Clinical data and comprehensive mitochondrial genomes were collected from the two families. Variants were assessed for evolutionary conservation, allelic frequencies, and their structural and functional impacts.
Characterization and phylogenetic analysis of the complete chloroplast genome of F. H. Chen & C. M. Hu (Asteraceae, Inuleae).
Mitochondrial DNA B Resour
December 2024
Ministry of Education, Key Laboratory of Land Resources Evaluation and Monitoring in Southwest (Sichuan Normal University), Chengdu, China.
In this study, we studied the complete chloroplast (cp) genome of F. H. Chen & C.
View Article and Find Full Text PDFThe plastid genome and phylogenetic status of C. Jeffrey & Y. L. Chen 1984 (Asteraceae).
Mitochondrial DNA B Resour
December 2024
College of Biology and Environmental Sciences, Jishou University, Jishou, Hunan, China.
The genus B. Nordenstam (1978) is a challenging taxonomic group with complex infrageneric relationships. Here, we newly report the plastid genome of S.
View Article and Find Full Text PDFThe complete chloroplast genome sequence of var. (Solanaceae), a new species of wolfberry from the Yellow River Basin in Ningxia, China.
Mitochondrial DNA B Resour
December 2024
Ningxia Technical College of Wine and Desertification Prevention, Yinchuan, Ningxia, China.
var. (2010), is a new variety of in Solanaceae. Here, we sequenced, assembled, and annotated the complete chloroplast (cp) genome of var.
View Article and Find Full Text PDFThe complete mitochondrial genome of an important medicinal plant, (Gaertn.) DC., 1845 (Lamiales, Orobanchaceae).
Mitochondrial DNA B Resour
December 2024
Shijiazhuang People's Medical College, Shijiazhuang, China.
, an extensively utilized Chinese herbal medicine, is highly valued for its medicinal properties. In this study, the complete mitochondrial genome (mitogenome) of was sequenced and assembled for the first time. The mitogenome is 547,032 bp in length, with an overall GC content of 44.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!