In the gas phase, some properties of native versus denatured protein conformations correspond to those in solution, such as affinity for protons and physical cross section. However, the capacity for hydrogen/deutrerium exchange is the opposite, with ubiquitin 7+ and 13+ ions exchanging >-60 D and approximately 15 D atoms, respectively. A variety of experimental methods now delineate a series of conformational perturbations that can occur in the 10(-12) s to 10(+2) s following electrospray, including side-chain collapse, hydrophobic and electrostatic non-covalent bond unfolding and refolding into a variety of non-native structures.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1255/ejms.1058 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Heteroleptic "open ruthenocene" for deposition of Ru films from the gas phase.
Dalton Trans
January 2025
Johnson Matthew Technology Centre, Blounts Court Rd., Sonning Common, RG4 9NH, UK.
A volatile heteroleptic open ruthenocene has been synthesised and characterised by NMR and single crystal X-ray diffraction. Using this compound as a precursor and oxygen as a co-reactant, a highly conductive Ru film has been deposited on Si with native oxide at 220 °C. Under the same deposition conditions, the film thickness obtained with the new compound has almost doubled compared to its homoleptic analogue.
View Article and Find Full Text PDFComputing chemical potentials with machine-learning-accelerated simulations to accurately predict thermodynamic properties of molten salts.
Chem Sci
January 2025
Chemical Sciences Division, Oak Ridge National Laboratory Oak Ridge TN 37830 USA
The successful design and deployment of next-generation nuclear technologies heavily rely on thermodynamic data for relevant molten salt systems. However, the lack of accurate force fields and efficient methods has limited the quality of thermodynamic predictions from atomistic simulations. Here we propose an efficient free energy framework for computing chemical potentials, which is the central free energy quantity behind many thermodynamic properties.
View Article and Find Full Text PDFPlant growth promotion via priming with volatile organic compounds emitted from strain EXTN-1.
Front Microbiol
January 2025
Research Institute of International Agriculture, Technology and Information, Hankyong National University, Anseong-si, Republic of Korea.
Volatile organic compounds (VOCs) produced by potential plant growth-promoting rhizobacteria (PGPR) play an important role in plant interactions. However, the mechanisms underlying this phenomenon are not well understood. Our findings show that the influence of VOCs from the PGPR strain (EXTN-1) on tobacco plant growth is dependent on the culture media used.
View Article and Find Full Text PDFiRGD-Targeted Biosynthetic Nanobubbles for Ultrasound Molecular Imaging of Osteosarcoma.
Int J Nanomedicine
January 2025
Department of Ultrasound, The second People's Hospital of Shenzhen, The First Affiliated Hospital of Shenzhen University, Shenzhen, 518061, People's Republic of China.
Purpose: Osteosarcoma is the most common primary malignant tumor of the bone. However, there is a lack of effective means for early diagnosis due to the heterogeneity of tumors and the complexity of tumor microenvironment. αvβ3 integrin, a crucial role in the growth and spread of tumors, is not only an effective biomarker for cancer angiogenesis, but also highly expressed in many tumor cells.
View Article and Find Full Text PDFWhen Photoelectrons Meet Gas Molecules: Determining the Role of Inelastic Scattering in Ambient Pressure X-ray Photoelectron Spectroscopy.
ACS Cent Sci
January 2025
Chemical Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.
Inelastic photoelectron scattering (IPES) by gas molecules, a critical phenomenon observed in ambient pressure X-ray photoelectron spectroscopy (APXPS), complicates spectral interpretation due to kinetic energy loss in the primary spectrum and the appearance of additional features at higher binding energies. In this study, we systematically investigate IPES in various gas environments using APXPS, providing detailed insights into interactions between photoelectrons emitted from solid surfaces and surrounding gas molecules. Core-level XPS spectra of Au, Ag, Zn, and Cu metals were recorded over a wide kinetic energy range in the presence of CO, N, Ar, and H gases, demonstrating the universal nature of IPES across different systems.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!