AI Article Synopsis
- A cell-based bone tissue engineering strategy shows promise for repairing large bone defects, emphasizing the importance of a reliable cell-seeding methodology for clinical applications.
- The study highlights that cell seeding efficiency (CSE) is crucial and can be affected by factors such as seeding medium-to-free scaffold volume ratio (MFR), seeding time, and scaffold morphology.
- Findings suggest that optimizing these parameters is necessary for improving CSE and ensuring consistent results in bone tissue engineering practices.
Article Abstract
The repair of large and complex bone defects could be helped by a cell-based bone tissue engineering strategy. A reliable and consistent cell-seeding methodology is a mandatory step in bringing bone tissue engineering into the clinic. However, optimization of the cell-seeding step is only relevant when it can be reliably evaluated. The cell seeding efficiency (CSE) plays a fundamental role herein. Results showed that cell lysis and the definition used to determine the CSE played a key role in quantifying the CSE. The definition of CSE should therefore be consistent and unambiguous. The study of the influence of five drop-seeding-related parameters within the studied test conditions showed that (i) the cell density and (ii) the seeding vessel did not significantly affect the CSE, whereas (iii) the volume of seeding medium-to-free scaffold volume ratio (MFR), (iv) the seeding time, and (v) the scaffold morphology did. Prolonging the incubation time increased the CSE up to a plateau value at 4 h. Increasing the MFR or permeability by changing the morphology of the scaffolds significantly reduced the CSE. These results confirm that cell seeding optimization is needed and that an evidence-based selection of the seeding conditions is favored.
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| http://dx.doi.org/10.1089/ten.TEC.2010.0069 | DOI Listing |
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