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High-pressure continuous culturing: life at the extreme.
Appl Environ Microbiol
January 2025
Department of Earth, Environmental and Planetary Sciences, Washington University in St. Louis, St. Louis, Missouri, USA.
Microorganisms adapted to high hydrostatic pressures at depth in the oceans and within the subsurface of Earth's crust represent a phylogenetically diverse community thriving under extreme pressure, temperature, and nutrient availability conditions. To better understand the microbial function, physiological responses, and metabolic strategies at conditions requires high-pressure (HP) continuous culturing techniques that, although commonly used in bioengineering and biotechnology applications, remain relatively rare in the study of the Earth's microbiomes. Here, we focus on recent developments in the design of HP chemostats, with particular emphasis on adaptations for delivery and sampling of dissolved gases.
View Article and Find Full Text PDFPhysiological relevance, localization and substrate specificity of the alternative (type II) mitochondrial NADH dehydrogenases of .
Front Microbiol
December 2024
Department of Biotechnology, Delft University of Technology, Delft, Netherlands.
Mitochondria from harbor a branched electron-transport chain containing a proton-pumping Complex I NADH dehydrogenase and three Type II NADH dehydrogenases (NDH-2). To investigate the physiological role, localization and substrate specificity of these enzymes, the growth of various NADH dehydrogenase knockout mutants was quantitatively characterized in shake-flask and chemostat cultures, followed by oxygen-uptake experiments with isolated mitochondria. NAD(P)H:quinone oxidoreduction of the three NDH-2 were individually assessed.
View Article and Find Full Text PDFSpecific growth rates and growth stoichiometries of Saccharomycotina yeasts on ethanol as sole carbon and energy substrate.
FEMS Yeast Res
January 2024
Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, the Netherlands.
Emerging low-emission production technologies make ethanol an interesting substrate for yeast biotechnology, but information on growth rates and biomass yields of yeasts on ethanol is scarce. Strains of 52 Saccharomycotina yeasts were screened for growth on ethanol. The 21 fastest strains, among which representatives of the Phaffomycetales order were overrepresented, showed specific growth rates in ethanol-grown shake-flask cultures between 0.
View Article and Find Full Text PDFAnalysis of early childhood intestinal microbial dynamics in a continuous-flow bioreactor.
Microbiome
December 2024
Genetics and Genome Biology, The Hospital for Sick Children, Toronto, ON, Canada.
Background: The human gut microbiota is inoculated at birth and undergoes a process of assembly and diversification during the first few years of life. Studies in mice and humans have revealed associations between the early-life gut microbiome and future susceptibility to immune and metabolic diseases. To resolve microbe and host contributing factors to early-life development and to disease states requires experimental platforms that support reproducible, longitudinal, and high-content analyses.
View Article and Find Full Text PDFQuantitative physiology and biomass composition of Cyberlindnera jadinii in ethanol-grown cultures.
Biotechnol Biofuels Bioprod
December 2024
Department of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629 HZ, Delft, The Netherlands.
Article Synopsis
- The study investigates how the yeast C. jadinii grows on ethanol as a carbon source, looking specifically at growth rates, energy requirements, and biomass composition across different culture methods.
- In ethanol-limited conditions, C. jadinii CBS 621 achieves effective biomass yields and demonstrates a stable protein content, even at low growth rates, indicating its potential for producing single-cell protein.
- The research also finds that various C. jadinii strains grow rapidly on ethanol, and the results from chemostat cultures can help model production outcomes in larger fed-batch systems, highlighting differences in protein content due to cultivation conditions.
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