Complete genome sequences were determined for two distinct strains of slow bee paralysis virus (SBPV) of honeybees (Apis mellifera). The SBPV genome is approximately 9.5 kb long and contains a single ORF flanked by 5'- and 3'-UTRs and a naturally polyadenylated 3' tail, with a genome organization typical of members of the family Iflaviridae. The two strains, labelled 'Rothamsted' and 'Harpenden', are 83% identical at the nucleotide level (94% identical at the amino acid level), although this variation is distributed unevenly over the genome. The two strains were found to co-exist at different proportions in two independently propagated SBPV preparations. The natural prevalence of SBPV for 847 colonies in 162 apiaries across five European countries was <2%, with positive samples found only in England and Switzerland, in colonies with variable degrees of Varroa infestation.
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http://dx.doi.org/10.1099/vir.0.022434-0 | DOI Listing |
Arch Virol
December 2024
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China.
Little is known about the insect viruses in wheat sawfly, Dolerus tritici, which is an important agricultural insect feeding on wheat leaves. Here, we used RNA sequencing to identify a novel single positive-strand RNA virus from the larvae of wheat sawfly collected in northern China and then determined its complete genome sequence by rapid amplification of cDNA ends. The complete genome is 9,594 nt in length, including a polyA tail at its 3' terminus, and it is predicted to encode a 326.
View Article and Find Full Text PDFDiabetes Obes Metab
February 2025
Department of Endocrinology, Singapore General Hospital, Singapore, Singapore.
Aim: Digital health interventions and economic incentives have shown promise in facilitating diabetes self-management, though evidence is limited. Therefore, this study aimed to evaluate the effectiveness and cost-effectiveness of a comprehensive app-based diabetes self-management programme with rewards for healthy behaviours and health outcomes.
Materials And Methods: The TRIal to slow the Progression Of Diabetes (TRIPOD) study was an open-label, parallel-group, randomised controlled trial conducted at Duke-NUS Medical School, Singapore.
Neurochem Res
November 2024
Department of Microbiology and Immunology, Medical University of South Carolina, 173 Ashley Avenue, Charleston, SC, 29425, USA.
Anim Microbiome
November 2024
Swine Research Group, Agroscope, 1725, Posieux, Switzerland.
Background: Specific sources of dietary fibres in sow gestation and lactation diets, such as inulin or wheat bran, have been shown to affect both the sow and its litter health by modulating the piglet's intestinal microbial population and composition. However, only a few studies have reported the effects of some specific fractions of the cell wall of the plants in the sow's lactation diet. Therefore, this study investigates the effect of increasing the level of HCs in a sow's lactation diet on the nutrient apparent total tract digestibility (ATTD), the faecal volatile fatty acid (VFA) profile, the microbiota of the sow and the microbiota and the performances of slow-growing (SG) and fast-growing (FG) piglets.
View Article and Find Full Text PDFJ Invertebr Pathol
September 2024
USDA-ARS, Bee Research Laboratory, 10300 Baltimore Avenue, Beltsville, MD 20705, USA. Electronic address:
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