Objective: To establish the fingerprint spectrum of the stems of Actinidia deliciosa by HPLC.
Methods: HPLC fingerprint analysis of the stems of Actinidia deliciosa was developed and the thermo Hypersil BDS C18 column (250 mm x 4.6 mm, 5 microm) was used. The mobile phase consisted of Acetonitril-0.1% phosphoric acid with gradient elution. The column temperature was 20 degrees C, the detective wavelength was 296 nm, the flow rate was 1.0 mL/min, and the sample injection was 20 microL.
Results: Fingerprint spectrum of the stems of Actinidia deliciosa was established, and 16 samples of different origin Actinidia deliciosa were detected. Twenty-eight peaks in chromatogram were common. There was a high similarity and each chromatographic peak was obtained with good separation correlation according to the technical requirements of fingerprint of Chinese traditional medicine.
Conclusion: This method is accurate, reliable and provides a scientific basis for controlling the quality of the stems of Actinidia deliciosa.
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