AI Article Synopsis
- RPL10 proteins are found widely in plants, with Arabidopsis having three types (RPL10A, RPL10B, RPL10C) and maize having two (rpl10-1 and rpl10-2), which are regulated by tissue type and development.
- Coimmunoprecipitation studies suggest Arabidopsis RPL10 proteins function as part of the 80S ribosome, and UV-B exposure affects their expression differently across species, showing some genes are upregulated while others are downregulated.
- Mutant analysis in Arabidopsis indicates that the RPL10 genes are not interchangeable; different mutations produce distinct phenotypes, highlighting their unique roles in plant development and translation during UV-B stress
Article Abstract
Ribosomal protein L10 (RPL10) proteins are ubiquitous in the plant kingdom. Arabidopsis (Arabidopsis thaliana) has three RPL10 genes encoding RPL10A to RPL10C proteins, while two genes are present in the maize (Zea mays) genome (rpl10-1 and rpl10-2). Maize and Arabidopsis RPL10s are tissue-specific and developmentally regulated, showing high levels of expression in tissues with active cell division. Coimmunoprecipitation experiments indicate that RPL10s in Arabidopsis associate with translation proteins, demonstrating that it is a component of the 80S ribosome. Previously, ultraviolet-B (UV-B) exposure was shown to increase the expression of a number of maize ribosomal protein genes, including rpl10. In this work, we demonstrate that maize rpl10 genes are induced by UV-B while Arabidopsis RPL10s are differentially regulated by this radiation: RPL10A is not UV-B regulated, RPL10B is down-regulated, while RPL10C is up-regulated by UV-B in all organs studied. Characterization of Arabidopsis T-DNA insertional mutants indicates that RPL10 genes are not functionally equivalent. rpl10A and rpl10B mutant plants show different phenotypes: knockout rpl10A mutants are lethal, rpl10A heterozygous plants are deficient in translation under UV-B conditions, and knockdown homozygous rpl10B mutants show abnormal growth. Based on the results described here, RPL10 genes are not redundant and participate in development and translation under UV-B stress.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2923885 | PMC |
| http://dx.doi.org/10.1104/pp.110.157057 | DOI Listing |
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