AI Article Synopsis
- Lymphocytes express nicotinic acetylcholine receptors (nAChR), but their expression on activated versus resting lymphocytes hasn't been studied.
- This research focused on comparing nAChR subunit expression in PHA-stimulated lymphocytes and leukemic cell lines, finding that only certain cells responded to nicotine with calcium waves.
- The findings indicate that PHA-stimulated lymphocytes show increased expression of the active form of the a-7 nAChR, suggesting that prior activation is necessary for these cells to respond to nicotine and release calcium.
Article Abstract
Lymphocytes are reported to express nicotinic acetylcholine receptors (nAChR). However, no data are available on the expression of these nAChR on activated lymphocyte relatively to resting lymphocytes. In this study, we examined nAChR subunits expression in PHA-stimulated versus un-stimulated lymphocytes, and four leukemic cell lines. Cell stimulation with nicotine triggered calcium responses only in some experiments conducted with PHA-stimulated lymphocytes. Likewise, only the Jurkat and HL-60 cell lines displayed calcium waves upon nicotine stimulation, whereas the Raji and CCRF-CEM did not. All responding cells displayed an active form of the nicotinic a-7 nAChR. Indeed, use of 2 different sets of primers for the corresponding mRNA showed that expression of the full-length a-7 subunit mRNA was only present in PHA-stimulated lymphocytes for which calcium waves had been evidenced. Microscopy analysis of lymphocytes structure showed a direct relationship between their size, their a-7 nAChR expression, and calcium release upon nicotine stimulation. Then, this relationship suggested that lymphocytes need a prime activation to express the a-7 nAChR, and therefore to release calcium in response to nicotine.
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