At similar HAI titers rabbit sera reveal a larger number of fractions (ten) in immunoelectrophoretic analysis of Sendai virus envelopes than do guinea pig sera, which only yield three distinct fractions. These differences are observed in both rocket and crossed immunoelectrophoresis, and are more marked in the latter. The number of fractions revealed does not differ in relation to the antigen used for hyperimmunization (intact Sendai virus, Triton-disrupted virus or envelope fractions extracted by Triton or Tween-ether). Solubilized Sendai virus envelopes obtained by Tween-ether or Triton treatment contain host antigens in their structure, the same as intact virus.
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