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Fad104, a positive regulator of adipogenesis, negatively regulates osteoblast differentiation. | LitMetric

Fad104, a positive regulator of adipogenesis, negatively regulates osteoblast differentiation.

Biochem Biophys Res Commun

Department of Molecular Biology, Graduate School of Pharmaceutical Science, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya, Aichi 467-8603, Japan.

Published: June 2010

AI Article Synopsis

  • Fad104 is a newly identified gene that plays a temporary role in the early stages of adipocyte (fat cell) differentiation, promoting this process in specific cell types, including mouse 3T3-L1 cells.
  • Research shows that cells lacking fad104 cannot properly differentiate into fat cells and instead form bone-like tissues, indicating fad104's involvement in osteoblast (bone cell) differentiation.
  • The study reveals that fad104 acts as a dual regulator: it promotes adipocyte differentiation while inhibiting osteoblast differentiation, with its expression decreasing during the differentiation of osteoblasts.

Article Abstract

Fad104 (factor for adipocyte differentiation 104) is a novel gene expressed temporarily in the early stages of adipocyte differentiation. Previously, we showed that fad104 promotes adipocyte differentiation in mouse 3T3-L1 cells and mouse embryonic fibroblasts (MEFs). Furthermore, we reported that implanted wild-type MEFs could develop into adipocytes, whereas fad104-deficient MEFs could not. Interestingly, bone-like tissues were only observed in the implants derived from fad104-deficient MEFs. This result implies that fad104 is involved in osteoblast differentiation. However, the functions of fad104 during osteogenesis are unknown. In this paper, we show that fad104 negatively regulates osteoblast differentiation. During the differentiation process, the level of fad104 expression decreased. Deletion of fad104 facilitated osteoblast differentiation in MEFs, and elevated the level of runx2, a master regulator of osteoblast differentiation. Disruption of fad104 suppressed BMP-2-mediated adipocyte differentiation in MEFs. In conclusion, we demonstrate that fad104 reciprocally regulates differentiation of adipocytes and osteoblast; functions as a positive regulator in adipocyte differentiation and as a negative regulator in osteoblast differentiation.

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Source
http://dx.doi.org/10.1016/j.bbrc.2010.05.077DOI Listing

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