AI Article Synopsis
- Hydroxyurea (HU) treatment is used to create a cell population with a higher percentage of cells in the G1 phase for improved testing of CD40 expression and IgG2a production upon stimulation with anti-mIgG.
- After applying HU, researchers also explored the effects of stimulating cells with Lipopolysaccharide (LPS) before and after treatment.
- Results showed that while HU can help enrich the G1 phase, it negatively impacts CD40 expression and IgG2a production induced by anti-mIgG, especially when LPS is used, diminishing its positive effects.
Article Abstract
Chemical treatment with hydroxyurea (HU) has been selected as a simple and low cost strategy to generate a cell population enriched for the G1 phase. After the chemical treatment with HU, cells were stimulated with anti-mIgG to test if the positive effects of anti-mIgG on CD40 expression and specific IgG2a production rate were improved upon a cell population with a higher percentage of cells in G1 phase at the beginning of the cell culture. In addition, other treatments assayed in this work were the cell stimulation with Lipopolysaccharide (LPS) both before and after the HU treatment. It has been observed that the use of HU under conditions able to maintain the cells in viable state (0.1 mM for 20 h), has a negative effect on CD40 expression and specific IgG2a production rate induced by anti-mIgG. The positive effect of LPS on cell stimulation induced by anti-mIgG is reduced on cells treated with HU.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2932903 | PMC |
| http://dx.doi.org/10.1007/s10616-010-9278-9 | DOI Listing |
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