Biological methods of wastewater treatment have been proved very effective for bioremediation of polluted sites. In this study, the degrading abilities of two bacteria Pseudomonas sp. and Staphylococcus xylosus, towards 1,2-dichlorobenzene (1,2-DCB), 2,4-dichlorophenol (2,4-DCP) and 4-Cl-m-cresol, are compared. Culture history and the presence of glucose as carbon source have been used for the optimization of cell's performance. 1,2-DCB showed the higher values of effective concentration (EC(50)), 1.04 and 0.84 mM with Pseudomonas sp. and S. xylosus respectively, whereas no substrate-inhibition appeared, in contrary to 4-Cl-m-cresol, that was more persistent in biodegradation by both bacteria. 2,4-DCP was less assimilated compared to 1,2-DCB, whereas bacterial specificity was higher, as it was found by the estimation of the half-saturation constant of 0.36 and 0.26 mM with Pseudomonas sp. and S. xylosus, respectively.
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