The automated method for enumeration of Escherichia coli, TEMPO EC, in foods uses a dehydrated culture medium and enumeration card containing 48 wells across three different dilutions for the automatic determination of the most probable number (MPN). The alternative method was compared in a multilaboratory collaborative study to AOAC Official Method 966.24. Six food types were artificially contaminated with E. coli: raw ground beef, bagged lettuce, cooked chicken, pasteurized crabmeat, frozen green beans, and pasteurized whole milk. All foods were analyzed for E. coli counts by 11 collaborating laboratories throughout the United States. Test portions from the six food types each contaminated at four different contamination levels were evaluated. The study demonstrated that the TEMPO EC method is a reliable, automated assay for the enumeration of E. coli in foods.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Identifying spatiotemporal patterns and drivers of fecal indicator bacteria in an urban lake for water quality assessment and management.
Heliyon
January 2025
Department of Environmental Sciences, Southern Illinois University Edwardsville, 44 Circle Drive SW 2145, PO Box 1099, Edwardsville, IL, USA, 62026.
The designated uses of lakes connect individuals to the natural environment, but some can expose recreational users to pathogens associated with fecal contamination that cause waterborne illnesses. Routine monitoring of fecal indicators in surface waters helps identify and track sources of fecal contamination to protect public health. We examined fecal indicators ( and enterococci) and factors influencing recreational freshwater quality.
View Article and Find Full Text PDFGenomic analysis, culturing optimization, and characterization of bacteriophage OSYSP, previously studied as effective pathogen control on fresh produce.
Front Microbiol
December 2024
Department of Food Science and Technology, The Ohio State University, Columbus, OH, United States.
Advances in bacteriophage genome sequencing and regulatory approvals of some bacteriophages in various applications have renewed interest in these antibacterial viruses as a potential solution to persistent food safety challenges. Here, we analyzed in depth the genome of the previously studied bacteriophage OSYSP (phage OSYSP), revealed its application-related characteristics, and optimized its enumeration techniques for facilitating industrial implementation. We previously sequenced phage OSYSP genome completely by combining results from Illumina Miseq and Ion Torrent sequencing platforms and completing the remaining sequence gaps using PCR.
View Article and Find Full Text PDFThe Effect of Heat-treated Poultry Pellets and Composted Poultry Litter on E. coli Survival in Southeastern US Soils: Florida and Georgia.
J Food Prot
January 2025
Food Science and Human Nutrition Department, Institute of Food and Agriculture Sciences, University of Florida, Gainesville, FL 32611, United States. Electronic address:
Biological soil amendments of animal origin (BSAAO) are a source of foodborne pathogens that can contaminate fresh produce. This study evaluated the survival of E. coli over 140 d in agricultural soils amended with composted poultry litter (PL), heat-treated poultry pellets (HTPP), or unamended (UN) in Florida (FL) and Georgia (GA).
View Article and Find Full Text PDFMicroscopic phage adsorption assay: High-throughput quantification of virus particle attachment to host bacterial cells.
Proc Natl Acad Sci U S A
December 2024
Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT 06520.
Phages, viruses of bacteria, play a pivotal role in Earth's biosphere and hold great promise as therapeutic and diagnostic tools in combating infectious diseases. Attachment of phages to bacterial cells is a crucial initial step of the interaction. The classic assay to quantify the dynamics of phage attachment involves coculturing and enumeration of bacteria and phages, which is laborious, lengthy, hence low-throughput, and only provides ensemble estimates of model-based adsorption rate constants.
View Article and Find Full Text PDFPredictors of household drinking water E. coli contamination: Population-based results from rural areas of Ghana, Malawi, Mozambique, Niger, Rwanda, Uganda, and Zambia.
Int J Hyg Environ Health
December 2024
The Water Institute, Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, 135 Dauer Drive, Chapel Hill, NC, 27599, USA. Electronic address:
Background: In sub-Saharan Africa, rural areas have lower rates of access to safe drinking water compared to urban areas. We investigated predictors of Escherichia coli contamination in drinking water of rural households in Ghana, Malawi, Mozambique, Niger, Rwanda, Uganda, and Zambia.
Methods: We used a population-based, cluster randomized sampling design to select rural households in each country.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!