A method is described for the entrapment of proteins in hydrazide-activated supports using oxidized glycogen as a capping agent. This approach is demonstrated using human serum albumin (HSA) as a model binding agent. After optimization of this method, a protein content of 43 (+/-1)mg of HSA/g support was obtained for porous silica. The entrapped HSA supports could retain a low-mass drug (S-warfarin) and had activities and equilibrium constants comparable to those for soluble HSA. It was also found that this approach could be used with other proteins and binding agents that had masses between 5.8 and 150kDa.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2900392 | PMC |
| http://dx.doi.org/10.1016/j.ab.2010.05.004 | DOI Listing |
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