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Instituto Israelita de Ensino e Pesquisa Albert Einstein, IIEPAE, São Paulo, Brazil.
Published: April 2010
AI Article Synopsis
The research presents a new method using ferromagnetic resonance (FMR) to quantify superparamagnetic iron oxide nanoparticles (SPIONs) in biological materials for both in vivo and in vitro studies.
In vivo, the study tracked how SPIONs were eliminated and distributed in Wistar rats after intravenous injection, with findings confirmed by X-ray fluorescence.
In vitro, the research quantified SPIONs attached to AC133 monoclonal antibodies to detect CD133 expression in stem cells from umbilical cord blood, demonstrating the effectiveness of FMR for measuring SPION concentrations.
Article Abstract
The aim of the present work is the presentation of a quantification methodology for the control of the amount of superparamagnetic iron oxide nanoparticles (SPIONs) administered in biological materials by means of the ferromagnetic resonance technique (FMR) applied to studies both in vivo and in vitro. The in vivo study consisted in the analysis of the elimination and biodistribution kinetics of SPIONs after intravenous administration in Wistar rats. The results were corroborated by X-ray fluorescence. For the in vitro study, a quantitative analysis of the concentration of SPIONs bound to the specific AC133 monoclonal antibodies was carried out in order to detect the expression of the antigenic epitopes (CD133) in stem cells from human umbilical cord blood. In both studies FMR has proven to be an efficient technique for the SPIONs quantification per volume unit (in vivo) or per labeled cell (in vitro).
