Objective: To identify genes regulated by HBV preS1-transactivated protein 2 binding protein 1 (PS1TP2BP1).
Methods: PS1TP2BP1 gene was amplified by polymerase chain reaction (PCR) technique and cloned into the eukaryotic expression vector pcDNA 3.1/my-c-His A. The mRNAs isolated from HepG2 cells transfected recombinant eukaryotic expression vector pcDNA 3.1/myc-HisA-PS1TP2BP1 and pcDNA 3.1/myc-HisA empty vector were used to construct subtractive library. The differentially expressed genes were identified and analyzed.
Results: 35 differentially expressed clones were obtained. Colony PCR identified 15 clones with 200-1000 bp inserts. Sequence analysis identified 15 differentially expressed genes.
Conclusion: This study provides data for further characterize the function of PS1TP2BP1.
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| http://dx.doi.org/10.3760/cma.j.issn.1007-3418.2010.04.008 | DOI Listing |
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