Pseudomonas fluorescens Pf1TZ was inhibitory in vitro to a number of phytopathogenic fungi and could protect vine plantlets against Botrytis cinerea. Total protection was reached after 3 weeks of bacterial inoculation. The endophytism of Pf1TZ was confirmed by confocal microscopy using its inherent fluorescence. The molecular characterization of Pf1TZ indicated the presence of genes from clusters encoding pyoluteorin and phenazine. The chromatographic purification and LC-MS(n) analysis revealed that the most active one has a molecular mass of 504 Da.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1007/s10529-010-0286-9 | DOI Listing |
Proc Natl Acad Sci U S A
January 2025
Department of Plant Pathology, Key Laboratory of Plant Immunity, Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Bacterial-fungal interaction (BFI) has significant implications for the health of host plants. While the diffusible antibiotic metabolite-mediated competition in BFI has been extensively characterized, the impact of intercellular contact remains largely elusive. Here, we demonstrate that the intercellular contact is a prevalent mode of interaction between beneficial soil bacteria and pathogenic filamentous fungi.
View Article and Find Full Text PDFMicrob Biotechnol
January 2025
Department of Chemistry, and Manchester Institute of Biotechnology (MIB), The University of Manchester, Manchester, UK.
Harnessing in situ microbial communities to clean-up polluted natural environments is a potentially efficient means of bioremediation, but often the necessary genes to breakdown pollutants are missing. Genetic bioaugmentation, whereby the required genes are delivered to resident bacteria via horizontal gene transfer, offers a promising solution to this problem. Here, we engineered a conjugative plasmid previously isolated from soil, pQBR57, to carry a synthetic set of genes allowing bacteria to consume terephthalate, a chemical component of plastics commonly released during their manufacture and breakdown.
View Article and Find Full Text PDFBiochem Biophys Res Commun
December 2024
Biophysics Institute, CNR-IBF, Via Corti 12, I-20133, Milano, Italy; Department of Bioscience, University of Milan, Via Celoria 26, I-20133, Milano, Italy. Electronic address:
Aldolases are crucial enzymes that catalyze the formation of carbon-carbon bonds in the context of the anabolic and catabolic pathways of various metabolites. The bacterium Pseudomonas fluorescens N3 can use naphthalene as its sole carbon and energy source by using, among other enzymes, the trans-o-hydroxybenzylidenepyruvate (tHBP) hydratase-aldolase (HA), encoded by the nahE gene. In this study, we present the crystallographic structures of tHBP-HA in three different functional states: the apo enzyme with a phosphate ion in the active site, and the Schiff base adduct bound either to pyruvate or to the substitute with '(R)-4-hydroxy-4-(2-hydroxyphenyl)-2-oxobutanoate'(intermediate state).
View Article and Find Full Text PDFMicrobiology (Reading)
January 2025
Department of Microbiology and Molecular Genetics, Larner College of Medicine, University of Vermont, Burlington, USA.
Sphingoid bases, including sphingosine, are important components of the antimicrobial barrier at epithelial surfaces where they can cause growth inhibition and killing of susceptible bacteria. is a common opportunistic pathogen that is less susceptible to sphingosine than many Gram-negative bacteria. Here, we determined that the deletion of the operon reduced growth in the presence of sphingosine.
View Article and Find Full Text PDFFood Res Int
January 2025
College of Food Science and Technology, Huazhong Agricultural University, National R & D Branch Center for Conventional Freshwater Fish Processing, Wuhan, Hubei Province 430070, PR China. Electronic address:
Photodynamic inactivation (PDI) has emerged as a novel non-thermal process technology for inactivating microorganisms due to its low cost, safety, and efficiency. This study aimed to investigate the antimicrobial effect of VK-mediated PDI against Pseudomonas fluorescens (P. fluorescens) and to assess its impact on the quality of the blunt bream contaminated with P.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!