AI Article Synopsis
- - The study aimed to investigate the relationship between genetic variations in the Plasmodium falciparum nhe-1 gene and the effectiveness of quinine as a treatment for malaria in a sample of 83 isolates from infected travelers in Africa and Madagascar.
- - Researchers identified 19 distinct genetic profiles of the nhe-1 gene, including 14 that were previously unknown, but found no significant link between these profiles and how susceptible the isolates were to quinine.
- - The only notable finding was that a specific genetic repeat pattern was associated with higher levels of quinine resistance, but overall, the polymorphisms tested did not serve as reliable indicators of quinine susceptibility in African isolates, leaving the potential for regional variations in other areas
Article Abstract
We sought to test the association of polymorphisms in Plasmodium falciparum nhe-1 (Pfnhe-1, gene PF13_0019) with in vitro susceptibility to quinine, which was previously reported in a limited number of reference strains or culture-adapted isolates. Determination of in vitro susceptibility to quinine, genotyping of Pfnhe-1 ms4760 microsatellite and polymorphism in codon 76 of Pfcrt were performed for 83 isolates obtained from symptomatic malaria-infected travelers returning from various African countries to France or from subjects living in Madagascar. Nineteen different ms4760 microsatellite profiles of Pfnhe-1 were found including 14 not previously described. Multivariate analysis showed no significant association between the in vitro susceptibility to quinine with particular ms4760 profiles. Contrary to previous reports, we only observed that the number of NHNDNHNNDDD repeats was positively associated with the increased IC50 of QN (P = 0.01). We concluded that the studied polymorphisms in Pfnhe-1 did not appear as valid molecular markers of in vitro susceptibility to quinine in P. falciparum isolates from Africa. Because we did not include any isolate of Asian origin in our series, these results did not exclude the possibility of regional associations, for example in South-East Asia.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2861372 | PMC |
| http://dx.doi.org/10.4269/ajtmh.2010.09-0327 | DOI Listing |
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