5.4 nm spatial resolution in biological photoemission electron microscopy.

Ultramicroscopy

Physics Department, Portland State University, 1719 SW 10th Avenue, Portland, OR 97201, USA.

Published: June 2010

We report a spatial resolution of 5.4 nm in images of sarcoplasmic reticulum from rabbit muscle. The images were obtained in an aberration-corrected photoemission electron microscope with a hyperbolic mirror as the correcting element for spherical and chromatic aberration. In-situ measurements and numerical simulations confirm the low residual aberration in the instrument and indicate the ultimate resolution in this type of microscopy to be below 2 nm.

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http://dx.doi.org/10.1016/j.ultramic.2010.04.005DOI Listing

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