AI Article Synopsis
- Coagulation factor VIII (FVIII) concentrates are crucial for treating Hemophilia A patients.
- Human FVIII was effectively purified from plasma using anion exchange chromatography and gel filtration methods, with varying recovery rates depending on the resin used.
- The purification process initially co-eluted vitamin K-dependent coagulation factors, but a subsequent step using Sepharose 6FF successfully recovered 70% of FVIII activity without those factors.
Article Abstract
Coagulation factor VIII (FVIII) concentrates are used in the treatment of patients with Hemophilia A. Human FVIII was purified directly from plasma using anion exchange chromatography followed by gel filtration. Three Q-Sepharose resins were tested, resulting in 40% recovery of FVIII activity using Q-Sepharose XL resin, about 80% using Q-Sepharose Fast Flow and 70% using the Q-Sepharose Big Beads. The vitamin K-dependent coagulation factors co-eluted with FVIII from the anion exchange columns. In the second step of purification, when Sepharose 6FF was used, 70% of FVIII activity was recovered free from vitamin K-dependent factors.
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| http://dx.doi.org/10.1007/s10529-010-0282-0 | DOI Listing |
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