AI Article Synopsis
- The study examined the role of different CD97 isoforms in gastric carcinoma using four cell lines, particularly focusing on BGC-823 cells with low CD97 expression.
- Over-expressing the CD97/EGF1,2,5 isoform enhanced the cells' motility and invasion, while increasing levels of this isoform led to a significant decrease in the full-length CD97/EGF1,2,3,4,5 isoform.
- The findings suggest that CD97 is linked to more advanced stages and greater invasiveness in gastric cancer, highlighting a potential tumor-promoting role for the small isoform and suppressive effects for the full-length isoform.
Article Abstract
The aim of this study was to elucidate the role of CD97 isoforms in gastric carcinoma. Out of four gastric cancer cell lines investigated, BGC-823 cells demonstrating low CD97 protein expression were stably transfected with pcDNA3.1 vector containing CD97/EGF1,2,5 or CD97/EGF1,2,3,4,5 inserts. Behavior of transfected cells was systematically investigated by employing proliferation, motility and invasive assays. As a result, we found that over-expression of CD97/EGF1,2,5 isoform correlated with increased motile and invasive ability of the clones. Furthermore, CD97/EGF1,2,5 isoform over-expression (3.8 times higher) was followed by significant decrease of CD97/EGF1,2,3,4,5 isoform (10.3 times lower). In contrast, CD97/EGF1,2,3,4,5 clones revealed significantly reduced invasive properties as compared with corresponding controls. The changes in acetylation status were one of the possible mechanisms affecting behavior of transfected cells. We concluded from the study that CD97 is closely related with advanced stages and higher invasiveness of gastric carcinoma. The study further lightened the tumor promoting role of CD97 small isoform in cancer progression and indicated the possible suppressive properties of the full length isoform of CD97.
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