Background & Objectives: Results of earlier studies to evaluate the possible role of complement system in tropical pulmonary eosinophilia (TPE) using classical methods like serum haemolyte component CH50, C3 and C4 levels were inconclusive. In this study we determined levels of serum C3d which is a catabolic fragment of C3, to find out any direct evidence of activation of the complement system in TPE.
Methods: The study population consisted of 3 groups. Group A consisted of 37 patients with well characterized TPE. In group B, 26 patients with pulmonary eosinophilia had similar respiratory and haemotological features as in Group A but had associated worm infestation in stool. The control group consisted of 39 healthy volunteers. Serum C3d levels were determined by sandwich ELISA technique.
Results: The serum C3d levels in TPE patients were not significantly different from those of the patients of group B or the normal controls.
Interpretation & Conclusions: Absence of significant change in serum C3d goes against the possibility of complement activation in TPE. Results of our study suggest that complement system is unlikely to play a pivotal role in pathogenesis of TPE.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Interrogating post-transplant donor HLA-specific antibody characteristics and effector functions using clinical bead assays.
Hum Immunol
November 2024
Department of Immunology, Duke University School of Medicine, Durham, NC, USA; Department of Surgery, Duke University School of Medicine, Durham, NC, USA. Electronic address:
Article Synopsis
- Single antigen bead (SAB) assays are the main technique for detecting donor-specific HLA antibodies after a transplant, but there's a lack of direct comparisons between traditional and modified methods for better monitoring.
- A study analyzed 251 post-transplant DSA from 91 serum samples using different treatments to determine the effectiveness of detecting antibodies; they found that some DSAs correlate with high IgG MFI values, indicating stronger antibody presence.
- The findings suggest that both the 1:16 dilution and C1q tests are effective indicators for detecting DSAs, with C1q positively correlating with total IgG levels, and modifications to SAB assays may help refine testing and interpretation of results
IgG4 glycosylation contributes to the pathogenesis of IgG4 Hashimoto's thyroiditis via the complement pathway.
Eur Thyroid J
October 2024
Department of Endocrinology, Peking University First Hospital, Xicheng District, Beijing, China.
Background: To explore whether IgG4 is involved in the pathogenesis of IgG4 HT.
Methods: Serum TgAb IgG4 and TPOAb IgG4 were measured in IgG4 HT and non-IgG4 HT. C1q, mannose-binding lectin (MBL), Bb, C3d, C4d, and membrane attack complex (MAC) in thyroid tissues from IgG4 HT, non-IgG4 HT, and controls were examined by immunohistochemistry.
Molecular mechanism of complement inhibition by the trypanosome receptor ISG65.
Elife
April 2024
Department of Biochemistry, University of Oxford, Oxford, United Kingdom.
Article Synopsis
- African trypanosomes live in infected mammals and face attacks from the immune system's complement system, particularly through a protein called C3, which targets pathogens for elimination.
- To evade this immune response, trypanosomes use a surface receptor called ISG65 that binds to C3b, which helps reduce the effectiveness of the immune system in clearing the infection.
- Research using cryogenic electron microscopy reveals that ISG65 has two binding sites for C3b and acts by forming a complex with C3b, blocking immune cell recruitment, and thereby protecting the trypanosomes from being eliminated.
A single-chain antibody construct with specificity of a natural IgM antibody reduces hepatic ischemia reperfusion injury in mice.
J Cell Mol Med
April 2024
Guangxi Key Laboratory of Molecular Medicine in Liver Injury and Repair, the Affiliated Hospital of Guilin Medical University, Guilin, Guangxi, China.
Natural immunoglobulin M (IgM) antibodies have been shown to recognize post-ischemic neoepitopes following reperfusion of tissues and to activate complement. Specifically, IgM antibodies and complement have been shown to drive hepatic ischemia reperfusion injury (IRI). Herein, we investigate the therapeutic effect of C2 scFv (single-chain antibody construct with specificity of a natural IgM antibody) on hepatic IRI in C57BL/6 mice.
View Article and Find Full Text PDFDiscovery and characterization of cross-reactive intrahepatic antibodies in severe alcoholic hepatitis.
Elife
December 2023
Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, United States.
Article Synopsis
- The study examined livers from 45 patients with severe alcoholic hepatitis (SAH) and found high levels of IgG and IgA antibodies linked to liver cell damage.
- Antibodies extracted from SAH livers showed the ability to kill liver cells and recognized specific human proteins not found in healthy livers or livers affected by other diseases.
- The research identified cross-reacting autoantibodies in SAH that target common cellular components, which is unique compared to antibodies from livers with other conditions.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!