AI Article Synopsis
- N(6)-Methyladenosine (m(6)A) is a common modified base found in the mRNA of higher eukaryotes, and its levels increase in yeast during sporulation, regulated by the methyltransferase Ime4, which is important for meiosis initiation.
- Research in Saccharomyces cerevisiae reveals significant m(6)A methylation in mRNA, particularly in the GpA context, indicating that about half of the transcripts could carry this modification during meiosis.
- A new antibody-based method was developed to identify m(6)A-containing transcripts, highlighting key meiosis regulators - IME1, IME2, and IME4 - with specific methyl
Article Abstract
N(6)-Methyladenosine (m(6)A) is a modified base present in the mRNA of all higher eukaryotes and in Saccharomyces cerevisiae, where there is an increase in m(6)A levels during sporulation. The methyltransferase, Ime4, is responsible for this modification and has a role in the initiation of meiosis. However, neither the function, nor the extent of distribution of this nucleotide modification is established. We demonstrate that in S. cerevisiae, substantial levels of internal adenosine methylation are present in the GpA context in mRNA from sporulating cells, which is consistent with the preferred methylation consensus of higher eukaryotes. Based upon our quantification data, every second transcript could contain one m(6)A during meiosis. As methylation is distributed across all mRNA size ranges, it is likely that m(6)A is not limited to a small population of messages. We developed a new antibody based method for identifying m(6)A containing messages, and using this method the transcripts of three key, early regulators of meiosis, IME1, IME2 and IME4 itself, were identified as being methylated. The position of m(6)A in IME2 was narrowed down to a region in the 3'-end. Methylation of these and other targets suggests mechanisms by which IME4 could control developmental choices leading to meiosis.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938207 | PMC |
| http://dx.doi.org/10.1093/nar/gkq266 | DOI Listing |
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