Site-directed mutagenesis in the study of membrane transporters.

Methods Mol Biol

Transporter Biology Group, Discipline of Pharmacology and Bosch Institute, University of Sydney, Sydney, NSW, Australia.

Published: July 2010

One of the major goals in membrane transporter research is to understand how transporter proteins work at the molecular level. Ideally, this research would be carried out with a detailed knowledge of the three-dimensional structure of the protein. However, in the absence of atomic resolution structures for many membrane transporters other molecular tools need to be employed. In vitro site-directed mutagenesis is one method that has the capacity to provide both structural information and identification of the role of individual residues and/or regions of a protein that are involved in function.

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http://dx.doi.org/10.1007/978-1-60761-700-6_15DOI Listing

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