We developed a method, MosDEL, to generate targeted knockouts of genes in Caenorhabditis elegans by injection. We generated a double-strand break by mobilizing a Mos1 transposon adjacent to the region to be deleted; the double-stranded break is repaired using injected DNA as a template. Repair can delete up to 25 kb of DNA and simultaneously insert a positive selection marker.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2878396 | PMC |
| http://dx.doi.org/10.1038/nmeth.1454 | DOI Listing |
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