Short-chain alcohol dehydrogenase, encoded by the gene Tsib_0319 from the hyperthermophilic archaeon Thermococcus sibiricus, was expressed in Escherichia coli, purified and characterized as an NADPH-dependent enantioselective oxidoreductase with broad substrate specificity. The enzyme exhibits extremely high thermophilicity, thermostability, and tolerance to organic solvents and salts.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2893467 | PMC |
| http://dx.doi.org/10.1128/AEM.02797-09 | DOI Listing |
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