[Correction of oxidative stress in the rat brain cortical cellular culture with vitamines E and C].

The oxidative stress-induced alteration in concentration of oxygen, lipid and nitrogen free radicals in cultured brain cortex of the newborn albino rats and its correction with vitamins E and C was investigated. Modeling the oxidative stress was achieved with addition of H(2)O(2) into the nutrient medium. In order to prevent an oxidative stress-induced cytotoxic effect, concomitantly with H(2)O(2), vitamins E and C were added into the nutrient medium. Oxygen, lipid and nitrogen free radicals were registered by method Electron Paramagnetic Resonance (EPR) and spin-traps. The data, obtained in our study revealed decreasing of intensity of NO content and increasing of spin trapped superoxid- (O(2-)) and lipoperoxid-radicals (LOO.) in explants of brain cortex of the newborn albino rats cultured in oxidative stress conditions. These changes were attenuated following action of vitamins E and C (decreasing of intensity of EPR signals of O2- and LOO, increasing of free NO content).