AI Article Synopsis
- A water-soluble dendrimer containing a maleimide-based fluorophore was synthesized, demonstrating bright fluorescence in CH(2)Cl(2) but weak fluorescence in water.
- The dendrimer exhibits relatively low cytotoxicity towards HeLa and A549 cell lines, showing reduced toxicity after 48 hours compared to 24 hours.
- Analysis of the dendrimer's association with plasmid DNA (BACE-GFP) suggests it may disrupt DNA's typical helical structure, with its binding strength evaluated through electrophoresis techniques.
Article Abstract
The synthesis of a water-soluble phosphorus-containing dendrimer possessing a fluorophore (maleimide-type) linked to the core is described. This dendrimer is found brightly fluorescent in CH(2)Cl(2), but poorly fluorescent in water. The cytotoxicity of this compound is relatively low towards HeLa and A549 cells, and less toxic after 48 hours than after 24 hours. Association of this dendrimer with plasmid DNA (BACE-GFP) analyzed with circular dichroism (CD) indicates a possible disturbing of the helical B-type structure of DNA. The strength of this association (a "dendriplex") with BACE-GFP (also with HygEGFP) was measured by electrophoresis.
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| http://dx.doi.org/10.1080/15257771003708462 | DOI Listing |
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