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Development of three additional Culex species-specific polymerase chain reaction primers and their application in West Nile virus surveillance in Canada. | LitMetric

Development of three additional Culex species-specific polymerase chain reaction primers and their application in West Nile virus surveillance in Canada.

J Am Mosq Control Assoc

Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2 Canada.

Published: March 2010

AI Article Synopsis

  • * Researchers developed specific PCR tests to identify different species of Culex mosquitoes and check for contamination with Culex DNA in non-Culex pools.
  • * The study found that about 6% of the non-Culex mosquito pools contained Culex DNA, highlighting the need for precautions to prevent cross-contamination in viral testing.

Article Abstract

In 2002, more than 17,000 mosquito pools collected in Canada (Quebec, Ontario, and Manitoba) were tested at the National Microbiology Laboratory in Winnipeg, Manitoba, for infection with West Nile virus (WNV). Using real-time reverse-transcriptase polymerase chain reaction (RT-PCR), 558 mosquito pools (86% Culex species and 14% other species) had evidence of infection with WNV. Only 30% of the Culex specimens, however, were identified to the species level. In this study, Culex species-specific PCR primers were designed to identify individual mosquitoes and mixed pools of Culex mosquitoes to species. In addition, pools of non-Culex mosquitoes that tested positive for WNV were also screened for Culex DNA to determine the frequency of cross-contamination among mosquitoes of different species. All DNA extracts from 121 Culex and 51 non-Culex pools, previously positive for WNV, were screened, and Culex DNA was detected in approximately 6% of non-Culex pools. This study demonstrates that contamination among mosquito species can occur and emphasizes that precautions should be taken to minimize this potentially confounding effect.

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Source
http://dx.doi.org/10.2987/09-5922.1DOI Listing

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